Scientists analyzing biotherapeutics are challenged to rapidly and robustly characterize an increasing number and wider variety of drug candidates in less time. Automated capillary electrophoresis instruments offer fast separation times and proven, high reproducibility for capillary sodium dodecyl sulfate gel electrophoresis (SDS-CGE), thus representing an excellent tool for the analysis of therapeutic proteins. However, due to the single capillary format of current systems, the throughput can be limited.
The SCIEX BioPhase 8800 system is a next generation instrument that utilizes a multi-capillary format that can assist in alleviating this issue, enabling protein characterization to be performed on multiple samples in parallel. In this poster, SDS-CGE analysis of multiple samples simultaneously to accelerate overall analysis time and potentially shorten product development workflows will be demonstrated. The BioPhase 8800 system additionally allows for analysis using UV and fluorescent detection in the same sequence, further reducing method development time.