Current strategies in lipidomic analysis to fully characterize a single lipid molecular species-- from sum composition to position and orientation of double bonds, generally requires several methodologies, lengthy extraction protocols, and complex separation techniques. To date, there is not a single injection solution to be able to provide a full structural snapshot of a complex lipid matrix. However, Electron Impact Excitation of Ions from Organics (EIEIO) has been recently reported on for lipid analysis and characterization of other singly charged molecules. Using EIEIO with an information dependent acquisition (IDA) experiment, we are able to identify and fully characterize complex lipid species in a biological matrix. A new methodology for the complete characterization of lipids is described that uses electron activated dissociation (EAD) on a ZenoTOF 7600 LC-MS/MS System. The rich MS/MS data produced by EAD contains unique fragment ions with patterns and intensities that can be used for complete lipid structure elucidation. Additionally, the EAD experiment is sensitive, reproducible, and fast, enabling on-the-fly characterization of lipids from complex mixtures during LC separation.